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Workshop on Biohydrogen`99

June 22 and 23, 1999, Tsukuba, Japan

A two-days meeting will be held with closed and round-table discussions by the selected experts of the field in the world. The aim of the meeting is to examine the status of the research and consider the future direction of the research after the international project of Biological production of Hydrogen by Environmentally Acceptable Technologies promoted by the Research Institute of Innovative Technology for the Earth (RITE). Additionally, we would like to discuss for the international cooperation between USA and Japan on this research field.
We are planning to edit an international book on biohydrogen with world-wide famous researchers in this year. We are happy if you bring a manuscript to contribute to the book. Your review article should be preferably considered for publication.


Organization
Organizer Dr. Jun Miyake
(National Institute for Advanced interdisciplinary Research)
Honorly Chair Prof. Tokio Ohta
(Yokohama National University)
Chair Japan Prof. Tadashi Matsunaga
(Tokyo University of Agriculture and Technology)
Chair USA Prof. Anthony San Pietro
(Indiana University)

Secretariat Dr. Masato Miyake
(National Institute of Bioscience and Human-Technology)
Higashi 1-1, Tsukuba, Ibaraki 305-8566, Japan.
Email: mmiyake@nibh.go.jp
Phone: +81 XXX XX 6052
Fax: +81 XXX XX 6139

Sponsorship
National Institute for Advanced Interdisciplinary Research


Program

21 June
(Common Room, 1F, ANNEX)

Reception and cocktail meeting 18:00-


22 June
(Training Room, 2F, ANNEX)

Opening remarks
Jun Miyake 9:00-9:10

1 Hydrogen Production
1-1 (Plenary lecture) Environmental problems and Biological H2
production.
Kazuhisa Miyamoto 9:10-9:35
1-2 Hydrogen production by photosynthetic bacteria: yields and
efficiencies.
Jorge Rocha, Maria Barbosa, and Rene Wijiffels 9:35-10:00
1-3 Hydrogen and poly-(hydroxyalkanoate) production from organic
acids by photosynthetis bacteria.
Ken Sasaki, Kenji Takeno, and Masanori Watanabe
10:00-10:25
1-4 Strategies for Improving oxygen tolerance of algal hydrogen
production.
Michael Seibert, Timothy Flynn, and Maria L. Ghirardi
10:25-10:50

2 Hydrogen World
2-1 Activity of HESS.
Yasukazu Saito 10:50-11:00
2-2 Current topics of hydrogen energy systems.
Tokio Ohta 11:00-11:20
2-3 Biohydrogen: An opportunity for international R&D
collaboration.
Anthony San Pietro and John Benemann 11:20-11:45
2-4 Hydrogenotrophy---microbiology from hydrogen----
Yasuo Igarashi 11:45-12:10

Lunch 12:10-13:30 (Common Room, 1F, ANNEX)

3 Biochemical and Metabolic Systems
3-1 (Plenary lecture) Maximizing Photosynthetic Productivities
and Hydrogen Production in Microalga Cultures
Juergen Polle, Saradadevi Kanakagiri, John R. Benemann and
Anastasios Melis 13:30-13:55
3-2 New aspects of the hydrogen metabolism of Scenedesmus
obliquus.
Horst Senger 13:55-14:20
3-3 A scheme for developing the yield of hydrogen at fermentation.
Shigeharu Tanisho 14:20-14:45

4 Molecular Biology
4-1 Cyanobacterial Biohydrogen: Present status and future
potential.
P. Lindblad and P. Tamagnini 14:45-15:10
4-2 Integration of hydrogen evolving systems with cellular
metabolism: The molecular biology and biochemistry of electron
transport factors and associated reductases.
Patrick C. Hallenbeck 15:10-15:35

Coffee brake 15:40-15:55 (Common Room, 1F, ANNEX)

5 Genetic Engineering

5-1 (Plenary lecture) Marine photosynthetic microorganisms for
hydrogen and chemical production.
Tadashi Matsunaga 15:55-16:20
5-2 Prediction of photosynthetic production rate of ethylene using
a recombinant cyanobacterium.
Takahira Ogawa 16:20-16:45
5-3 Genetic engineering for cyanobacterial hydrogen production.
M. Miyake, J. Schnackenberg, Y. Koike, J. Miyake,
and Y. Asada 16:45-17:10

Banquet 18:00-20:00 (The Rural House Kisse)

You can enjoy a tipical architecture of rural house in Ibaraki prefecture. We have a dinner with a music of a Japanese harp "Koto" in "The Rural House Kisse".
We are going to the Rural House Kisse by bus. The bus will pick you up at 17:20 in front of Sakura-Kan.


23 June
(Training Room, 2F, ANNEX)

6 Reactors
6-1 (Plenary lecture) Production of hydrogen by an Anabaena mutant
in a photobioreactor under aerobic, outdoor conditions.
D. O. Hall, K. K. Rao, A. S. Fedorov, and A. A. Tsygankov
9:00-9:25
6-2 Hydrogen photoproduction by purple bacteria. Immobilized vs
suspension cultures.
A. A. Tsygankov 9:25-9:50
6-3 Biological hydrogen production as an environmentally friendly
technology.
RITE research group (K. Kiyama) 9:50-10:15
6-4 Designing photobioreactors for photobiological production of
hydrogen.
James C. Ogbonna and Hideo Tanaka 10:15-10:40
6-5 Hydrogen production from food processing wastewater and sewage
sludge by anaerobic dark fermentation combined with photo-
fermentation.
Mi-Sun Kim 10:40-11:05

Poster with coffee 11:05-12:00 (Common Room, 1F, ANNEX)

Lunch 12:10-13:10 (Common Room, 1F, ANNEX)

7 Discussion for Future 13:10-14:45
Discussion Leaders: T. Matsunaga, A. San Pietro, and
J. Miyake

8 Conclusion
T. Matsunaga 14:45-15:00

Laboratory tour 15:00-


24 June

Farewell

Poster presentations
(You can mount the materials after resistration on 21 June.)

P-1 Photobiological hydrogen production, nitrogenase and hydrogenase
activities in come cyanobacteria.
H. Masukawa, K. Nakamura, M. Mochimaru, and H. Sakurai

P-2 Construction of Escherichia coli uptake hydrogenase mutants and its
impact on fermentation pathway.
Shinsuke Yamamoto, Masamitsu Tomiyama, and Koji Sode

P-3 Hydrogen Production from Industrial Wastewater by Anaerobic
Microflora.
Yoshiyuki Ueno, Seiji Otsuka, Youji Kitajima,
and Masafumi Goto

P-4 Hydrogen Production by Anoxygenic Phototrophic Bacteria
in the presence of NH4+.
Heguang Zhu, Tatsuki Wakayama, Shunsaku Ueda, Tomoo Suzuki,
Yasio Asada, and Jun Miyake

P-5 The properties of heat treatment liquor extract of sewage
sludge as raw material for hydrogen production by photosynthetic
bacteria Rb. sphaeroides strain RV.
Masaaki Kimura and Noboru Shirakawa

P-6 Hydrogen production by photosynthetic bacteria :
Light source and wavelength effect on the conversion efficiency
of light to hydrogen.
Eiju NAKADA, Tatuki WAKAYAMA and Jun MIYAKE

P-7 Effect of light/dark cycle on bacterial hydrogen production by
Rhodobacter sphaeroides RV -from hour to second region-.
Tatsuki Wakayama and Jun Miyake

P-8 Analysis of Light Compensation Point Under Various Conditions
of Bioreactor using Photosynthetic Bacteria.
Y. Kitajima, Y. Ueno, I. Sakamoto, M. Goto, S. Otsuka

P-9 In vitro and in vivo coupling of Thiocapsa hydrogenase with
cyanobacterial and algal electron mediators.
J. Schnackenberg, M. Miyake, J. Miyake, N. A. Zorin,
and Y. Asada

P-10 Heterologous Expression of Clostridial [Fe-only]Hydrogenase in
Cyanobacterium Synechococcus sp. PCC7942
Y.Koike, J.Schnackenberg, M.Miyake, J.Miyake, and Y.Asada

P-11 HERBICIDE SENSOR USING PHOTOSYNTHETIC REACTION CENTER
C. Nakamura, K. Shimada, M. Hasegawa, M. Shirai and J. Miyake

P-12 CYTOCHROME C3-LB FILM PREPARED BY USING AN AMPHIPHILIC PEPTIDE
FOR HYDROGEN EVOLVING DEVICE
K. Noda, C. Nakamura, N. A. Zorin, H. Akutsu and J. Miyake

P-13 PORPHYRIN DETECTION BY USING PEPTIDE LIGAND AND SPR
C. Nakamura, Y. Inuyama, K. Shirai, S. Nakano, N. Sugimoto and
J. Miyake

P-14 Prevention of Marine Biofouling by Electrochemical Methods
Noriyuki Nakamura, Tsuruo Nakayama, Hitoshi Wake, Mina Okochi,
Tadashi Matsunaga

P-15 Entropy Engineering
Jun Miyake, Momoko Nagata, and Toshinori Kusumi